Anticorps Recombinant de lapin anti-ADNP

ADNP Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242687C11

N° de cat : 85054-2-PBS

Synonymes

Activity-dependent neuroprotective protein, Activity-dependent neuroprotector homeobox protein, ADNP1, KIAA0784

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Galerie de données de validation

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  WB analysis using 85054-2-RR (same clone as 85054-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85054-2-RR (ADNP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85054-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85054-2-RR (same clone as 85054-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ADNP antibody (85054-2-RR, Clone: 242687C11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85054-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 85054-2-RR (same clone as 85054-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ADNP Recombinant antibody (85054-2-RR, Clone:242687C11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85054-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01783-2

  Cytometric bead array standard curve of MP01783-2, ADNP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85054-2-PBS. Detection antibody: 85054-1-PBS. Standard: Ag12414. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP01783-1

  Cytometric bead array standard curve of MP01783-1, ADNP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85054-2-PBS. Detection antibody: 85054-3-PBS. Standard: Ag12414. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 85054-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85054-2-RR against Human ADNP were performed. The affinity constant is below 1 pM.

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Informations sur le produit

85054-2-PBS cible ADNP dans les applications de WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ADNP Protéine recombinante Ag12414
• Nom complet: activity-dependent neuroprotector homeobox
• Masse moléculaire calculée: 1102 aa, 123 kDa
• Poids moléculaire observé: 135-145 kDa
• Numéro d’acquisition GenBank: BC075794
• Symbole du gène: ADNP
• Identification du gène (NCBI): 23394
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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