Anticorps Polyclonal de lapin anti-Adenosine A1 Receptor

Adenosine A1 Receptor Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 20332-1-AP

Synonymes

Adenosine receptor A1, ADORA1, RDC7

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Galerie de données de validation

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  WB analysis of mouse eye using 20332-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 20332-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20332-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 20332-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 20332-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of SH-SY5Y using 20332-1-AP

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Adenosine A1 Receptor Polyclonal antibody (20332-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu oculaire de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

20332-1-AP cible Adenosine A1 Receptor dans les applications de WB, IHC, IF, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Adenosine A1 Receptor Protéine recombinante Ag14092
• Nom complet: adenosine A1 receptor
• Masse moléculaire calculée: 326 aa, 37 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC026340
• Symbole du gène: Adenosine A1 Receptor
• Identification du gène (NCBI): 134
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADORA1, also named as RDC7, is a receptor for adenosine. It is mediated by G proteins which inhibit adenylyl cyclase. Adenosine is an important mediator of ethanol intoxication and exerts some of its effects via ADORA1 in the central nervous system.

Publications

Species	Application	Title
mouse	IHC	Cell Biol Toxicol; Adenosine kinase protects against acetaminophen-induced acute liver injury by activating autophagy in hepatocytes; Authors - Chuanxin Zhang
human	FC (Intra)	PLoS One; Discovery of a potent, selective, and tumor-suppressing antibody antagonist of adenosine A2A receptor; Authors - Linya Wang
mouse	WB,IF	J Orthop Translat; CD73 alleviates osteoarthritis by maintaining anabolism and suppressing catabolism of chondrocytes extracellular matrix; Authors - Hu Guo
human	WB	Am J Physiol Cell Physiol; Abnormal purine metabolism in nasal epithelial cells affects allergic rhinitis by regulating Th17/Treg cells; Authors - Ting Xu

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