Anticorps Polyclonal de lapin anti-Adenosine A1 Receptor

Adenosine A1 Receptor Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 55026-1-AP

Synonymes

Adenosine A1 Receptor, Adenosine receptor A1, ADORA1, RDC7

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 55026-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55026-1-AP (Adenosine A1 Receptor antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 55026-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 55026-1-AP (Adenosine A1 Receptor antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 55026-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 55026-1-AP (Adenosine A1 Receptor antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 55026-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 55026-1-AP (Adenosine A1 Receptor antibody at dilution of 1:50.

• 

  IHC staining of mouse testis using 55026-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 55026-1-AP (Adenosine A1 Receptor antibody at dilution of 1:50.

• 

  IHC staining of mouse brain using 55026-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55026-1-AP (Adenosine A1 Receptor antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55026-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55026-1-AP (Adenosine A1 Receptor antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y
• Résultats positifs en IHC: tissu de cervelet de souris, tissu cérébral de souris, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

55026-1-AP cible Adenosine A1 Receptor dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: adenosine A1 receptor
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: NM_000674
• Symbole du gène: ADORA1
• Identification du gène (NCBI): 134
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Adenosine A1 receptor (ADORA1, also known as A1AR) is one of four subtypes of adenosine receptors that belong to a superfamily of protein G-coupled receptors. Adenosine is produced in response to trauma, ischemia, and hypoxia and plays an important role in preventing excessive tissue damage. 

What is the molecular weight of adenosine A1 receptor? Are there any isoforms of ADORA1?

The molecular weight of adenosine A1 receptor is 32-37 kDa. ADORA1 forms homodimers that can be stable in denaturing condition running as a ~64 kDa species in western blotting (PMID: 19558453). Alternative splicing resulting in two isoforms has been reported (PMID: 8300646). 

What is the tissue expression of adenosine A1 receptor? What is the subcellular localization of ADORA1?

Adenosine A1 receptor is expressed in various organs, with very high expression in the central nervous system (mainly in the cortex, cerebellum, and hippocampus but also by microglia, astrocytes, and oligodendrocytes), adrenal gland, and atrium (PMID: 11734617). Adenosine receptors, including A1 receptor, are integral membrane proteins that reside at the plasma membrane. 

What is the role of adenosine A1 receptor in health and disease?

Adenosine is an important neuromodulator influencing synaptic plasticity and the release of neurotransmitters, in addition to working as a neuroprotective agent against oxidative stress - all of these functions are mediated by adenosine A1 receptor, whose expression levels are increased upon stress. Adenosine A1 receptor has been shown to control sleep; ADORA1 levels increase during sleep deprivation and promote sleep (PMID: 9157887, 17329439). Additionally, adenosine A1 receptor regulates vascular tension by contraction, antagonizing the activity of adenosine A2 receptor (PMID: 15539423). A number of selective agonists and antagonists of adenosine receptors have been developed to study their biology and are now drug candidates in the clinic (PMID: 19639277).

Publications

Species	Application	Title
human	WB	Sci Transl Med; Effects of caffeine on the human circadian clock in vivo and in vitro.; Authors - Tina M Burke
human,rat	WB	Antioxidants (Basel); Activation of Adenosine A1 Receptor in Ischemic Stroke: Neuroprotection by Tetrahydroxy Stilbene Glycoside as an Agonist.; Authors - Lingyu Ruan
mouse	WB	J Nutr Biochem; Disruption of hepatic one-carbon metabolism impairs mitochondrial function and enhances macrophage activity in methionine-choline-deficient mice.; Authors - Brandon J Eudy
human,mouse	WB	Bioorg Med Chem; Unraveling the mechanism of alkaloids from Sophora alopecuroides Linn combined with immune checkpoint blockade in the treatment of non-small cell lung cancer based on systems pharmacology.; Authors - Sen Chen
mouse	IHC,IF	Anat Rec (Hoboken); Adenosine A1 Receptor mRNA Expression by Neurons and Glia in the Auditory Forebrain.; Authors - Troy A Hackett
human		J Ophthalmic Vis Res; Adenosine Receptors Expression in Human Retina and Choroid with Age-related Macular Degeneration; Authors - Collin P Goebel

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Brandon (Verified Customer) (10-31-2019)

40 ug protein was transferred to a PVDF membrane and blocked in 5% milk in TBST for 1 hour before overnight incubation with 1' antibody diluted in 1% milk in TBST. Anti-rabbit HRP secondary antibody diluted 1:3000 in 5% milk was used for detection by chemiluminescence.The band did not show up when transferred to a nitrocellulose membrane and I noticed several non-specific bands

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: THP-1 macrophages

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