Anticorps Polyclonal de lapin anti-ADORA2A

ADORA2A Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 51092-1-AP

Synonymes

adenosine A2a receptor, Adenosine receptor A2a, ADORA2, ADORA2A, hA2aR, RDC8

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Galerie de données de validation

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  WB analysis of mouse brain using 51092-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 51092-1-AP (ADORA2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 51092-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 51092-1-AP (ADORA2A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 51092-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 51092-1-AP (ADORA2A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 51092-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51092-1-AP (ADORA2A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 51092-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51092-1-AP (ADORA2A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

51092-1-AP cible ADORA2A dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADORA2A Protéine recombinante Ag0470
• Nom complet: adenosine A2a receptor
• Masse moléculaire calculée: 412 aa, 45 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC013780
• Symbole du gène: ADORA2A
• Identification du gène (NCBI): 135
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Adenosine (ADO) is a retaliatory metabolite that is expressed in conditions of injury or stress. Its physiological functions are mediated through interaction with four specific transmembrane receptors called ADORA1, ADORA2A, ADORA2B, and ADORA3 (PMID:23994158). ADORA2A (adenosine A2A receptor) has been shown to dampen inflammatory responses. ADORA2A signaling has been discovered on polymorphonuclear neutrophils (PMNs) (PMID:36009485).

Publications

Species	Application	Title
rat	WB	Mol Neurobiol; Ketamine counteracts sevoflurane-induced depressive-like behavior and synaptic plasticity impairments through the adenosine A2A receptor/ERK pathway in rats; Authors - Weiwei Yu
rat	WB,IHC	Pediatr Res; Caffeine treatment started before injury reduces hypoxic-ischemic white-matter damage in neonatal rats by regulating phenotypic microglia polarization.; Authors - Liu Yang
human	WB	Ophthalmic Res; 7-Methylxanthine Influences the Behavior of ADORA2A-DRD2 Heterodimers in Human Retinal Pigment Epithelial Cells.; Authors - Yuzhuo Fan
mouse		Adv Sci (Weinh); Targeting the Negative Feedback of Adenosine-A2AR Metabolic Pathway by a Tailored Nanoinhibitor for Photothermal Immunotherapy.; Authors - Yiqiong Liu
mouse	WB	J Pharm Anal; Inosine: a broad-spectrum anti-inflammatory against SARS-CoV-2 infection-induced acute lung injury via suppressing TBK1 phosphorylation; Authors - Ningning Wang
human	WB	Front Pediatr; Analysis and comparisons of gene expression changes in patient- derived neurons from ROHHAD, CCHS, and PWS; Authors - A Kaitlyn Victor