Anticorps Monoclonal anti-ADPGK

ADPGK Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B4B8

N° de cat : 68034-1-PBS

Synonymes

2610017G09Rik, ADP dependent glucokinase, ADP GK, ADPGK, DKFZp434B195, RbBP 35

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Galerie de données de validation

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  WB analysis using 68034-1-Ig (same clone as 68034-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68034-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 68034-1-Ig (same clone as 68034-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68034-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 68034-1-Ig (same clone as 68034-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68034-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68034-1-Ig (same clone as 68034-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68034-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 68034-1-Ig (same clone as 68034-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68034-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 68034-1-Ig (same clone as 68034-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68034-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68034-1-PBS cible ADPGK dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ADPGK Protéine recombinante Ag31242
• Nom complet: ADP-dependent glucokinase
• Masse moléculaire calculée: 497 aa, 54 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC006112
• Symbole du gène: ADPGK
• Identification du gène (NCBI): 83440
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ADP-dependent glucokinase (ADPGK) has frst been described 1994 in hyperthermophilic archaea as a novel glucose-phosphorylating enzyme dependent on ADP (adenosine diphosphate) instead of ATP (adenosine triphosphate). Highest ADPGK expression is found in immune cells of both myeloid and lymphoid lineages. Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency (By similarity).

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