Anticorps Polyclonal de lapin anti-ADRA1A-Specific

• Phare
• Validé par KD/KO

ADRA1A-Specific Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19777-1-PBS

Synonymes

ADRA1A, Alpha-1C adrenergic receptor, Alpha-1A adrenoreceptor, Alpha-1A adrenoceptor, Alpha-1A adrenergic receptor

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Galerie de données de validation

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  WB analysis using 19777-1-AP (same clone as 19777-1-PBS)

  mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 19777-1-AP (ADRA1A-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours without or with blocking peptide (1.4 ug peptide was used to block the binding of 1.0 ug antibody). This data was developed using the same antibody clone with 19777-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 19777-1-AP (same clone as 19777-1-PBS)

  WB result of ADRA1A-Specific antibody (19777-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADRA1A-Specific transfected HeLa cells. This data was developed using the same antibody clone with 19777-1-PBS in a different storage buffer formulation.

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  WB analysis using 19777-1-AP (same clone as 19777-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19777-1-AP (ADRA1A-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19777-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 19777-1-AP (same clone as 19777-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19777-1-AP (ADRA1A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19777-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 19777-1-AP (same clone as 19777-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ADRA1A-Specific Polyclonal antibody (19777-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 19777-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19777-1-PBS cible ADRA1A-Specific dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: adrenergic, alpha-1A-, receptor
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: NM_000680
• Symbole du gène: ADRA1A
• Identification du gène (NCBI): 148
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ADRA1A, also named as ADRA1C, belongs to the G-protein coupled receptor 1 family. ADRA1A mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. The antibody is specific to ADRA1A.

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