Anticorps Polyclonal de lapin anti-ADRB2

ADRB2 Polyclonal Antibody for WB, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 29864-1-AP

Synonymes

ADRB2R, ADRBR, B2AR, BAR, Beta 2 adrenergic receptor

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Galerie de données de validation

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  WB analysis using 29864-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 29864-1-AP (ADRB2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 29864-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 29864-1-AP (ADRB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse stomach using 29864-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using ADRB2 antibody (29864-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549,
• Résultats positifs en IF-P: tissu d'estomac de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

29864-1-AP cible ADRB2 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADRB2 Protéine recombinante Ag32313
• Nom complet: adrenergic, beta-2-, receptor, surface
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46-48 kDa
• Numéro d’acquisition GenBank: BC012481
• Symbole du gène: ADRB2
• Identification du gène (NCBI): 154
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Beta-2 adrenergic receptor (ADRB2), a member of the G protein-coupled receptor (GPCR) family, binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. ADRB2 is directly linked to one of its final effectors, the class C L-type Ca2+ channel, Cav1.2 (PMID: 11441182). This receptor-channel complex also contains a G protein, an adenylyl cyclase, cyclic adenosine monophosphate-dependent protein kinase (PKA), and a counteracting phosphatase, PP2A. Different polymorphic forms, point mutations, and/or downregulation of the gene of ADRB2 are associated with nocturnal asthma, obesity, and type 2 diabetes. This antibody detects ADRB2 with a molecular weight of 46-48 kDa as has been shown by some researches (PMID: 30542705; 35075132).

Publications

Species	Application	Title
mouse,human	WB	iScience; Region-specific sympatho-adrenergic regulation of specialized vasculature in bone homeostasis and regeneration; Authors - Hao-Kun Xu
human	WB	Oncol Lett; β‑adrenergic receptor activation promotes the proliferation of HepG2 cells via the ERK1/2/CREB pathways; Authors - Xingcheng Lin
mouse	WB	Immunobiology; Exploring liquid-liquid phase separation-related diagnostic biomarkers in osteoarthritis based on machine learning algorithms and experiment; Authors - Bo Dong
mouse,human	IHC	Adv Sci (Weinh); Chronic Stress-Induced and Tumor Derived SP1+ Exosomes Polarizing IL-1β+ Neutrophils to Increase Lung Metastasis of Breast Cancer; Authors - Leyi Zhang
	WB	Nat Cell Biol; Psychological stress-induced ALKBH5 deficiency promotes tumour innervation and pancreatic cancer via extracellular vesicle transfer of RNA; Authors - Ziming Chen
human	WB	Front Pharmacol; Gut microbiota-derived metabolite phenylacetylglutamine inhibits the progression of prostate cancer by suppressing the Wnt/β-catenin signaling pathway; Authors - Jing Lv

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