Anticorps Recombinant de lapin anti-Perilipin-2

Perilipin-2 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

230274F4

N° de cat : 80362-2-RR

Synonymes

230274F4, ADFP, Adipophilin, Adipose differentiation-related protein, ADRP

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Galerie de données de validation

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  WB analysis using 80362-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80362-2-RR (Perilipin-2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 80362-2-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 80362-2-RR (ADRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 80362-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80362-2-RR (ADRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 80362-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 80362-2-RR (ADRP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 80362-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 80362-2-RR (ADRP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 80362-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP antibody (80362-2-RR, Clone: 230274F4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  IF Staining of HeLa using 80362-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP antibody (80362-2-RR, Clone: 230274F4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  IF Staining of HeLa using 80362-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP antibody (80362-2-RR, Clone: 230274F4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HepG2 using 80362-2-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ADRP Recombinant antibody (80362-2-RR, Clone:230274F4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 80362-2-RR

  Biolayer interferometry (BLl) kinetic assays of 80362-2-RR against Human ADRP were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules K-562, cellules THP-1, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa traitées à l'acide oléique,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

80362-2-RR cible Perilipin-2 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Perilipin-2 Protéine recombinante Ag7539
• Nom complet: adipose differentiation-related protein
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC005127
• Symbole du gène: Perilipin-2
• Identification du gène (NCBI): 123
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.

Publications

Species	Application	Title
	WB	Am J Physiol Cell Physiol; Inhalation Exposure to Airborne PM2.5 Attenuates Hepatic Metabolic Pathways through S-Nitrosylation of the Primary ER Stress Sensor; Authors - Zhao Yang

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