Anticorps Recombinant de lapin anti-Perilipin-2

Perilipin-2 Recombinant Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230274F4

N° de cat : CL488-80362-2

Synonymes

ADRP/Perilipin 2, ADRP, ADFP, Perilipin 2, 230274F4

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Galerie de données de validation

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  IF Staining of HeLa using CL488-80362-2

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using CoraLite® Plus 488 ADRP antibody (CL488-80362-2, Clone: 230274F4 ) at dilution of 1:200, CL594-phalloidin (red).

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  FC experiment of HepG2 using CL488-80362-2

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 ADRP Recombinant Antibody (CL488-80362-2, Clone:230274F4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa traitées à l'acide oléique,
• Résultats positifs en FC (Intra): cellules HepG2

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-80362-2 cible Perilipin-2 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Perilipin-2 Protéine recombinante Ag7539
• Nom complet: adipose differentiation-related protein
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC005127
• Symbole du gène: Perilipin-2
• Identification du gène (NCBI): 123
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.

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