Anticorps Polyclonal de lapin anti-AEBP2

AEBP2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 11232-2-AP

Synonymes

Adipocyte enhancer-binding protein 2, AE-binding protein 2, AEBP 2

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Galerie de données de validation

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  WB analysis of human testis using 11232-2-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11232-2-AP (AEBP2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11232-2-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11232-2-AP (AEBP2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 11232-2-AP

  IP result of anti-AEBP2 (IP:11232-2-AP, 4ug; Detection:11232-2-AP 1:300) with mouse testis tissue lysate 4800ug.

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain
• Résultats positifs en IP: tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• ChIP: See 1 publications below

Informations sur le produit

11232-2-AP cible AEBP2 dans les applications de WB, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AEBP2 Protéine recombinante Ag1756
• Nom complet: AE binding protein 2
• Masse moléculaire calculée: 33 kDa, 54 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC015624
• Symbole du gène: AEBP2
• Identification du gène (NCBI): 121536
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AEBP2, also named as Zinc finger protein AEBP2 or Adipocyte enhancer-binding protein 2, is a 517 amino acid protein, which contains 3 C2H2-type zinc fingers and belongs to the AEBP2/jing C2H2-type zinc-finger family. AEBP2 as a DNA-binding transcriptional repressor may interact with and stimulate the activity of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3. It may e playing a role in craniofacial and digital development, as well as development of the central nervous system and gastrointestinal tract.

Publications

Species	Application	Title
mouse	WB	Nature; Dual functions of Tet1 in transcriptional regulation in mouse embryonic stem cells.; Authors - Wu Hao H
human	WB	Mol Cancer; Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer; Authors - Rashmi Bharti
human		PLoS Genet; Alu Elements in ANRIL Non-Coding RNA at Chromosome 9p21 Modulate Atherogenic Cell Functions through Trans-Regulation of Gene Networks.; Authors - Holdt Lesca M LM
mouse	WB,ChIP	Nucleic Acids Res; AEBP2 as a potential targeting protein for Polycomb Repression Complex PRC2.; Authors - Kim Hana H
mouse	WB	PLoS One; Aebp2 as an epigenetic regulator for neural crest cells.; Authors - Kim Hana H

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