Anticorps Polyclonal de lapin anti-AFAP1

• Phare
• Validé par KD/KO

AFAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (1)

N° de cat : 21093-1-AP

Synonymes

110 kDa actin filament-associated protein, Actin filament-associated protein 1, AFAP, AFAP 110, AFAP-110

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Galerie de données de validation

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  WB analysis using 21093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21093-1-AP (AFAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21093-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21093-1-AP (AFAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 21093-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21093-1-AP (AFAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 21093-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21093-1-AP (AFAP1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 21093-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21093-1-AP (AFAP1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 21093-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using AFAP1 antibody (21093-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 21093-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AFAP1 antibody (21093-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules HeLa, cellules MCF-7, cellules PC-3, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 1 publications below

Informations sur le produit

21093-1-AP cible AFAP1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AFAP1 Protéine recombinante Ag15346
• Nom complet: actin filament associated protein 1
• Masse moléculaire calculée: 730 aa, 81 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC032777
• Symbole du gène: AFAP1
• Identification du gène (NCBI): 60312
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Actin filament-associated protein 1 (AFAP1) encodes a motor fiber-associated protein, which organizes a network for linking other proteins, including SRC proto-oncogene, non-receptor tyrosine kinase (Src) and protein kinase C . A long non-coding RNA, namely AFAP1-antisense RNA 1 (AS1), is transcribed from the antisense strand of this gene and potentially regulates its expression.(PMID: 31086663, PMID: 35193469)

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; CSNK2B modulates IRF1 binding to functional DNA elements and promotes basal and agonist-induced antiviral signaling; Authors - Moe Matsumoto ; KD Validated
	WB,IF	Mol Biol Cell; Spatial proximity of proteins surrounding zyxin under force-bearing conditions.; Authors - Joleen S Cheah ; KD Validated
rat	WB	Int J Mol Med; Curculigoside attenuates myocardial ischemia‑reperfusion injury by inhibiting the opening of the mitochondrial permeability transition pore.; Authors - Yanbing Zhao
human	WB	Biol Res; Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer.; Authors - Xu Han
human	WB	J Exp Clin Cancer Res; LncRNA AFAP1-AS1 promotes tumorigenesis and epithelial-mesenchymal transition of osteosarcoma through RhoC/ROCK1/p38MAPK/Twist1 signaling pathway.; Authors - Deyao Shi
human	WB	Oncotarget; LncRNA AFAP1-AS1 promotes growth and metastasis of cholangiocarcinoma cells.; Authors - Xiuhui Shi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (05-04-2019)

Works excellently for WB. Strong signal by 5s exposure and with typical dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

AFAP1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:21093-1-AP)

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