Anticorps Polyclonal de lapin anti-AFAP1L2

• Phare
• Validé par KD/KO

AFAP1L2 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 17183-1-AP

Synonymes

AFAP1 like protein 2, AFAP1L2, KIAA1914, XB130

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Galerie de données de validation

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  WB analysis using 17183-1-AP

  A549 were subjected to SDS PAGE followed by western blot with 17183-1-AP (AFAP1L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 17183-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17183-1-AP (AFAP1L2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 17183-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17183-1-AP (AFAP1L2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 17183-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17183-1-AP (AFAP1L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse thymus using 17183-1-AP

  IP Result of anti-AFAP1L2 (IP:17183-1-AP, 4ug; Detection:17183-1-AP 1:500) with mouse thymus tissue lysate 2800ug.

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  IHC staining of human thymus using 17183-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 17183-1-AP (AFAP1L2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thymus using 17183-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 17183-1-AP (AFAP1L2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, tissu de thymus de souris
• Résultats positifs en IP: tissu de thymus de souris
• Résultats positifs en IHC: tissu de thymus humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

17183-1-AP cible AFAP1L2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AFAP1L2 Protéine recombinante Ag10966
• Nom complet: actin filament associated protein 1-like 2
• Masse moléculaire calculée: 818 aa, 91 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC033212
• Symbole du gène: AFAP1L2
• Identification du gène (NCBI): 84632
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AFAP1L2, also named as Actin filament-associated protein 1-like 2 or XB130, is a 818 amino acid protein, which contains two PH domains. AFAP1L2 is detected in spleen and thyroid, and at lower levels in kidney, brain, lung and pancreas. AFAP1L2 localizes in the cytoplasm and may play a role in a signaling cascade by enhancing the kinase activity of SRC. AFAP1L2 promotes thyroid tumor growth, and its expression in these cancer cells may affect cell proliferation and survival by controlling the expression of multiple genes, especially transcription regulators.

Publications

Species	Application	Title
human	IHC	Transl Oncol; Identification of genes associated with local aggressiveness and metastatic behavior in soft tissue tumors.; Authors - Cunha Isabela Werneck IW
mouse	IHC	Carcinogenesis; XB130 deficiency enhances carcinogen-induced skin tumorigenesis.; Authors - Hae-Ra Cho ; KO Validated
mouse	WB	Mol Cell; PLEKHS1 drives PI3Ks and remodels pathway homeostasis in PTEN-null prostate; Authors - Tamara A M Chessa
human	WB	Oncol Lett; The 3'‑untranslated region of XB130 regulates its mRNA stability and translational efficiency in non‑small cell lung cancer cells; Authors - Qinrong Wang
mouse	IF,WB,IHC	Cell Rep; Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease; Authors - Min Yu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tamara (Verified Customer) (12-02-2020)

antibody works very well in detecting endogenous Afap1l2 protein in mouse prostate lysates

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse prostate