Anticorps Polyclonal de lapin anti-AFF4

• Phare
• Validé par KD/KO

AFF4 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14662-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 14662-1-AP (same clone as 14662-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14662-1-AP (AFF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14662-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14662-1-AP (same clone as 14662-1-PBS)

  IP result of anti-AFF4 (IP:14662-1-AP, 4ug; Detection:14662-1-AP 1:20000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 14662-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14662-1-PBS cible AFF4 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AFF4 Protéine recombinante Ag6336
• Nom complet: AF4/FMR2 family, member 4
• Masse moléculaire calculée: 127 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC063007
• Symbole du gène: AFF4
• Identification du gène (NCBI): 27125
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Transcriptional controled by RNA polymerase II (Pol II) is a multi-step process requiring the concerted action of multiple factors and contacts with the DNA template for the proper synthesis of nascent RNA [PMID:12676794]. ELL1 was demonstrated to be a Pol II elongation factor capable of enhancing the catalytic rate of transcription elongation by reducing transient pausing by the enzyme [PMID:8596958]. AFF4 was initially identified as a fusion partner of the MLL (mixed-lineage leukemia) gene participated in infant acute lymphoblastic leukemia, and a component of the ELL/p-TEFb elongation complex. AFF4 interacts with positive transcription elongation factor-b while also repressing Tat transactivation of HIV-1 [PMID:17389929].

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