Anticorps Monoclonal anti-AFG3L2

AFG3L2 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C12F3

N° de cat : 68517-1-PBS

Synonymes

Mitochondrial inner membrane m-AAA protease component AFG3L2, EC:3.4.24.-, AFG3-like protein 2, AFG3L 2, AFG3 like protein 2

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Galerie de données de validation

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  WB analysis using 68517-1-Ig (same clone as 68517-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68517-1-Ig (AFG3L2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68517-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 68517-1-Ig (same clone as 68517-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AFG3L2 antibody (68517-1-Ig, Clone: 1C12F3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68517-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68517-1-Ig (same clone as 68517-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug AFG3L2 Monoclonal antibody (68517-1-Ig, Clone:1C12F3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68517-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68517-1-PBS cible AFG3L2 dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AFG3L2 Protéine recombinante Ag7180
• Nom complet: AFG3 ATPase family gene 3-like 2 (yeast)
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC065016
• Symbole du gène: AFG3L2
• Identification du gène (NCBI): 10939
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly(PMID:17101804). Human AFG3L2 is an 80-kDa protein encoded by a 17-exon gene and highly and selectively expressed in human cerebellar Purkinje cells(PMID:20208537) and it can exsit as a truncated 65 kDa protein(PMID:18337413). Defects in AFG3L2 are the cause of spinocerebellar ataxia type 28 (SCA28) and spastic ataxia autosomal recessive type 5 (SPAX5).

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