Anticorps Monoclonal anti-AFM

AFM Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G9F6

N° de cat : 66113-1-PBS

Synonymes

ALF, ALBA, ALB2, afamin/AFM, afamin

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Galerie de données de validation

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  WB analysis of human plasma using 66113-1-Ig (same clone as 66113-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66113-1-Ig (AFM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

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  IP experiment of human plasma using 66113-1-Ig (same clone as 66113-1-PBS)

  IP result of anti-AFM (IP:66113-1-Ig, 5ug; Detection:66113-1-Ig 1:500) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66113-1-Ig (same clone as 66113-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66113-1-Ig (AFM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66113-1-Ig (same clone as 66113-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66113-1-Ig (AFM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66113-1-Ig (same clone as 66113-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66113-1-Ig (AFM Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66113-1-Ig (same clone as 66113-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66113-1-Ig (AFM Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66113-1-Ig (same clone as 66113-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AFM antibody (66113-1-Ig, Clone: 2G9F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66113-1-PBS cible AFM dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AFM Protéine recombinante Ag16866
• Nom complet: afamin
• Masse moléculaire calculée: 599 aa, 69 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC109020
• Symbole du gène: AFM
• Identification du gène (NCBI): 173
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.

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