Anticorps Recombinant de lapin anti-AFP

AFP Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

230435A2

N° de cat : 82982-5-RR

Synonymes

Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin

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Galerie de données de validation

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  WB analysis of HepG2 using 82982-5-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 82982-5-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82982-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82982-5-RR (AFP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 82982-5-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AFP antibody (82982-5-RR, Clone: 230435A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 82982-5-RR

  Biolayer interferometry (BLl) kinetic assays of 82982-5-RR against Human AFP were performed. The affinity constant is 0.696 nM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HuH-7, placenta humain
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82982-5-RR cible AFP dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: alpha-fetoprotein
• Masse moléculaire calculée: 69 kDa
• Poids moléculaire observé: 68-72 kDa
• Numéro d’acquisition GenBank: BC027881
• Symbole du gène: AFP
• Identification du gène (NCBI): 174
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)

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