Anticorps Polyclonal de lapin anti-AGAP2

AGAP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14690-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human brain using 14690-1-AP (same clone as 14690-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 14690-1-AP (same clone as 14690-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 14690-1-AP (same clone as 14690-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 14690-1-AP (same clone as 14690-1-PBS)

  IP result of anti-AGAP2 (IP:14690-1-AP, 4ug; Detection:14690-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14690-1-AP (same clone as 14690-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14690-1-AP (AGAP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14690-1-AP (same clone as 14690-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14690-1-AP (AGAP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14690-1-AP (same clone as 14690-1-PBS)

  Immunofluorescent analysis of Hela cells, using AGAP2 antibody 14690-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14690-1-PBS cible AGAP2 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AGAP2 Protéine recombinante Ag6142
• Nom complet: ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
• Masse moléculaire calculée: 125 kDa
• Poids moléculaire observé: 124 kDa
• Numéro d’acquisition GenBank: BC028020
• Symbole du gène: AGAP2
• Identification du gène (NCBI): 116986
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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