Anticorps Polyclonal de lapin anti-AGER/RAGE

• Phare
• Validé par KD/KO

AGER/RAGE Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(51)

N° de cat : 16346-1-AP

Synonymes

RAGE, AGER, Advanced glycosylation end product-specific receptor

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Galerie de données de validation

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  WB analysis using 16346-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16346-1-AP (AGER/RAGE antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 16346-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16346-1-AP (AGER/RAGE antibody) at dilution of 1:4000 (under 10x lens).

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  IHC staining of mouse lung using 16346-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16346-1-AP (AGER/RAGE antibody) at dilution of 1:4000 (under 40x lens).

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  IHC staining of human lung using 16346-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 16346-1-AP (AGER antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  IHC staining of human renal cell carcinoma using 16346-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 16346-1-AP (AGER Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 16346-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 16346-1-AP (AGER Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung using 16346-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 16346-1-AP (AGER antibody) at dilution of 1:4000 (under 10x lens).

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  IF Staining of mouse lung using 16346-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using AGER/RAGE antibody (16346-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, tissu pulmonaire de rat
• Résultats positifs en IHC: tissu pulmonaire de souris, tissu de carcinome à cellules rénales humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu pulmonaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 38 publications below
• IHC: See 10 publications below
• IF: See 11 publications below

Informations sur le produit

16346-1-AP cible AGER/RAGE dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AGER/RAGE Protéine recombinante Ag9540
• Nom complet: advanced glycosylation end product-specific receptor
• Masse moléculaire calculée: 404 aa, 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC020669
• Symbole du gène: AGER
• Identification du gène (NCBI): 177
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a member of the immunoglobulin superfamily of cell surface receptors, which interacts with distinct families of ligands, mediating diverse functions in a broad array of cell types including cellular migration, proliferation, survival and apoptosis (PMID: 12645002; 17425919). It senses endogenous stress signals with a broad ligand repertoire including advanced glycation end products, S100 proteins, high-mobility group box 1 protein/HMGB1, amyloid beta/APP oligomers, nucleic acids, phospholipids and glycosaminoglycans (PMID: 19910580; 28627626). It interacts with distinct molecules implicated in homeostasis, development, inflammation, and certain diseases such as diabetes and Alzheimer's disease (PMID: 26253613; 31079281).

Publications

Species	Application	Title
	IF	EMBO J; A novel human fetal lung-derived alveolar organoid model reveals mechanisms of surfactant protein C maturation relevant to interstitial lung disease; Authors - Kyungtae Lim
Rat		Oxid Med Cell Longev; l-Theanine Ameliorates d-Galactose-Induced Brain Damage in Rats via Inhibiting AGE Formation and Regulating Sirtuin1 and BDNF Signaling Pathways.; Authors - Li Zeng
rat	IF,WB	J Neuroinflammation; Inhibiting HMGB1-RAGE axis prevents pro-inflammatory macrophages/microglia polarization and affords neuroprotection after spinal cord injury.; Authors - Hong Fan
mouse	WB	J Neuroinflammation; Celastrol exerts a neuroprotective effect by directly binding to HMGB1 protein in cerebral ischemia-reperfusion.; Authors - Dan-Dan Liu
human,mouse	WB,IHC	Mol Med; AGEs promote atherosclerosis by increasing LDL transcytosis across endothelial cells via RAGE/NF-κB/Caveolin-1 pathway; Authors - Meng Shu ; KD Validated
mouse	WB	Front Pharmacol; Protective effect of heat-processed Gynostemma pentaphyllum on high fat diet-induced glucose metabolic disorders mice; Authors - Jin-Bo Xie

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