Anticorps Monoclonal anti-AGER/RAGE

AGER/RAGE Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

3D4G11

N° de cat : 66833-1-PBS

Synonymes

AGER, RAGE, 3D4G11, Advanced glycosylation end product-specific receptor

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Galerie de données de validation

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  WB analysis of pig lung using 66833-1-Ig (same clone as 66833-1-PBS)

  pig lung tissue were subjected to SDS PAGE followed by western blot with 66833-1-Ig (AGER antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

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  WB analysis of rat lung using 66833-1-Ig (same clone as 66833-1-PBS)

  rat lung tissue were subjected to SDS PAGE followed by western blot with 66833-1-Ig (AGER antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 66833-1-Ig (same clone as 66833-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66833-1-Ig (AGER antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 66833-1-Ig (same clone as 66833-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66833-1-Ig (AGER antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse lung using 66833-1-Ig (same clone as 66833-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using AGER/RAGE antibody (66833-1-Ig, Clone: 3D4G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse lung using 66833-1-Ig (same clone as 66833-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using AGER/RAGE antibody (66833-1-Ig, Clone: 3D4G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat lung using 66833-1-Ig (same clone as 66833-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat lung tissue using AGER antibody (66833-1-Ig, Clone: 3D4G11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66833-1-PBS cible AGER/RAGE dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AGER/RAGE Protéine recombinante Ag9657
• Nom complet: advanced glycosylation end product-specific receptor
• Masse moléculaire calculée: 404 aa, 43 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC020669
• Symbole du gène: AGER
• Identification du gène (NCBI): 177
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a multiligand receptor of the immunoglobulin superfamily. It interacts with distinct molecules implicated in homeostasis, development, and inflammation, and certain diseases such as diabetes and Alzheimer disease.

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