Anticorps Monoclonal anti-AGR2

AGR2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1A8A8

N° de cat : 66768-1-Ig

Synonymes

GOB 4, AGR 2, AG-2, AG2, AG 2

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Galerie de données de validation

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  WB analysis of pig stomach using 66768-1-Ig

  pig stomach tissue were subjected to SDS PAGE followed by western blot with 66768-1-Ig (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 66768-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 66768-1-Ig (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 66768-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66768-1-Ig (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66768-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66768-1-Ig (AGR2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66768-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66768-1-Ig (AGR2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human colon cancer using 66768-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human colon cancer using 66768-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of A549 using 66768-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:1500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004).

• 

  IF Staining of HT-29 using 66768-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HT-29 using 66768-1-Ig

  1X10^6 HT-29 cells were intracellularly stained with 0.2 ug Anti-Human AGR2 (66768-1-Ig, Clone:1A8A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), and 0.2 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Applications testées

• Résultats positifs en WB: tissu d'estomac de porc, cellules HT-29, cellules T-47D
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du côlon humain,
• Résultats positifs en IF/ICC: cellules HT-29, cellules A549
• Résultats positifs en FC (Intra): cellules HT-29,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

66768-1-Ig cible AGR2 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AGR2 Protéine recombinante Ag2919
• Nom complet: anterior gradient homolog 2 (Xenopus laevis)
• Masse moléculaire calculée: 175 aa, 20 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC015503
• Symbole du gène: AGR2
• Identification du gène (NCBI): 10551
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used to aide in assessment of high-risk patients undergoing endoscopic procedures.

Publications

Species	Application	Title
human	WB	Mol Cancer; LINC02273 drives breast cancer metastasis by epigenetically increasing AGR2 transcription.; Authors - Bingqiu Xiu
human	WB	Front Oncol; QKI-6 Suppresses Cell Proliferation, Migration, and EMT in Non-Small Cell Lung Cancer.; Authors - Haihua Zhang

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