Anticorps Monoclonal anti-AGR2

AGR2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A8A8

N° de cat : 66768-1-PBS

Synonymes

GOB 4, AGR 2, AG-2, AG2, AG 2

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Galerie de données de validation

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  WB analysis of pig stomach using 66768-1-Ig (same clone as 66768-1-PBS)

  pig stomach tissue were subjected to SDS PAGE followed by western blot with 66768-1-Ig (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

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  WB analysis of T-47D using 66768-1-Ig (same clone as 66768-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 66768-1-Ig (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 66768-1-Ig (same clone as 66768-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66768-1-Ig (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66768-1-Ig (same clone as 66768-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66768-1-Ig (AGR2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66768-1-Ig (same clone as 66768-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66768-1-Ig (AGR2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 66768-1-Ig (same clone as 66768-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 66768-1-Ig (same clone as 66768-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 66768-1-Ig (same clone as 66768-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:1500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

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  IF Staining of HT-29 using 66768-1-Ig (same clone as 66768-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

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  FC experiment of HT-29 using 66768-1-Ig (same clone as 66768-1-PBS)

  1X10^6 HT-29 cells were intracellularly stained with 0.2 ug Anti-Human AGR2 (66768-1-Ig, Clone:1A8A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), and 0.2 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66768-1-PBS cible AGR2 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AGR2 Protéine recombinante Ag2919
• Nom complet: anterior gradient homolog 2 (Xenopus laevis)
• Masse moléculaire calculée: 175 aa, 20 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC015503
• Symbole du gène: AGR2
• Identification du gène (NCBI): 10551
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used to aide in assessment of high-risk patients undergoing endoscopic procedures.

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