Anticorps Recombinant de lapin anti-AGRN

AGRN Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251295C12

N° de cat : 86507-3-PBS

Synonymes

Agrin, Agrin C-terminal 110 kDa subunit, Agrin C-terminal 22 kDa fragment, Agrin C-terminal 90 kDa fragment, Agrin N-terminal 110 kDa subunit

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Galerie de données de validation

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  WB analysis using 86507-3-RR (same clone as 86507-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86507-3-RR (AGRN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86507-3-PBS in a different storage buffer formulation.

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Informations sur le produit

86507-3-PBS cible AGRN dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: AGRN Protéine recombinante Eg5123
• Nom complet: agrin
• Masse moléculaire calculée: 215 kDa
• Poids moléculaire observé: 245 kDa
• Numéro d’acquisition GenBank: NM_198576.4
• Symbole du gène: AGRN
• Identification du gène (NCBI): 375790
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Agrin, a large heparan sulfate proteoglycan, is expressed in neuronal, as well as nonneuronal tissues (PMID:12671652). Nerve-derived agrin has been demonstrated to play an essential role in development and maintenance of the neuromuscular junction (PMID:16386415). The agrin gene encodes a protein of more than 2000 amino acids with predicted molecular weight of 225 kDa but can display as 600 kDa protein due to extensive N-terminal glycosylation (PMID:12671652). Neurotrypsin cleaves the proteoglycan agrin at two homologous, highly conserved sites, liberating a middle 90-kDa and a C-terminal 22-kDa fragment from the membrane or ECM-bound N-terminal moiety of agrin (PMID:18230682).

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