Anticorps Monoclonal anti-Angiotensinogen/AGT

Angiotensinogen/AGT Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B2F5

N° de cat : 68020-1-PBS

Synonymes

AGT, Angiotensinogen, Serpin A8, SERPINA8, 1B2F5

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Galerie de données de validation

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  WB analysis of human plasma using 68020-1-Ig (same clone as 68020-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 68020-1-Ig (same clone as 68020-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HuH-7 using 68020-1-Ig (same clone as 68020-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 68020-1-Ig (same clone as 68020-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig plasma using 68020-1-Ig (same clone as 68020-1-PBS)

  pig plasma were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 68020-1-Ig (same clone as 68020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 68020-1-Ig (same clone as 68020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 68020-1-Ig (same clone as 68020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 68020-1-Ig (same clone as 68020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 68020-1-Ig (same clone as 68020-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AGT antibody (68020-1-Ig, Clone: 1B2F5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68020-1-PBS cible Angiotensinogen/AGT dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Angiotensinogen/AGT Protéine recombinante Ag16658
• Nom complet: angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• Masse moléculaire calculée: 485 aa, 53 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC011519
• Symbole du gène: AGT
• Identification du gène (NCBI): 183
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene also have been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

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