Anticorps Polyclonal de lapin anti-AGTR1

• Phare
• Validé par KD/KO

AGTR1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(70)

Avis (2)

N° de cat : 25343-1-AP

Synonymes

AG2S, AGTR1A, AGTR1B, Angiotensin II type 1 receptor, Angiotensin II type-1 receptor

---

Galerie de données de validation

• 

  WB analysis using 25343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25343-1-AP (AGTR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 25343-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25343-1-AP (AGTR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 25343-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 25343-1-AP (AGTR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25343-1-AP (AGTR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 25343-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25343-1-AP (AGTR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 25343-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25343-1-AP (AGTR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 25343-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25343-1-AP (AGTR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 25343-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25343-1-AP (AGTR1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 25343-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25343-1-AP (AGTR1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 25343-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25343-1-AP (AGTR1 Antibody) at dilution of 1:50 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: cellules C2C12, cellules HEK-293, cellules HuH-7, cellules PC-12, tissu cardiaque de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 56 publications below
• IHC: See 17 publications below
• IF: See 14 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

25343-1-AP cible AGTR1 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AGTR1 Protéine recombinante Ag14461
• Nom complet: angiotensin II receptor, type 1
• Masse moléculaire calculée: 359 aa, 41 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC022447
• Symbole du gène: AGTR1
• Identification du gène (NCBI): 185
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Angiotensin II (Ang II), the main effector molecule of the renin-angiotensin system, exerts its actions mainly via interaction with type-1 angiotensin II receptor (AGTR1, also named AT1R), thereby contributing to blood pressure regulation. AGTR1 mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. By regulating vascular tone, cardiovascular function, and salt and water homeostasis, AGTR1 exerts an indispensable physiological role (PMID: 21600887). AGTR1 has been implicated in diverse aspects of human disease, from the regulation of blood pressure and cardiovascular homeostasis to cancer progression (PMID: 26975580).

Publications

Species	Application	Title
mouse,human	WB,CoIP	Cell Res; Cartilage oligomeric matrix protein is an endogenous β-arrestin-2-selective allosteric modulator of AT1 receptor counteracting vascular injury.; Authors - Yi Fu
human	WB	Adv Sci (Weinh); Transcriptomics-Based Liquid Biopsy for Early Detection of Recurrence in Locally Advanced Gastric Cancer; Authors - Ping'an Ding ; KD Validated
mouse	WB	Aging Cell; Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling; Authors - Ruili Dang
human	IF	Biomaterials; Inhibition of tumor-promoting stroma to enforce subsequently targeting AT1R on tumor cells by pathological inspired micelles.; Authors - Yun Zhu
mouse	WB	Redox Biol; Vitamin D receptor activation regulates microglia polarization and oxidative stress in spontaneously hypertensive rats and angiotensin II-exposed microglial cells: Role of renin-angiotensin system.; Authors - Changmeng Cui
rat,mouse	WB,IF	Free Radic Biol Med; Spinal AT1R contributes to neuroinflammation and neuropathic pain via NOX2-dependent redox signaling in microglia; Authors - Wencui Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Kazu (Verified Customer) (12-14-2022)

This antibody worked well on mouse optic nerve sections at optimized dilution of 1:100.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Optic nerve

FH

Wu (Verified Customer) (07-16-2021)

Works good for IHC assay

• Applications: Immunohistochemistry
• Cell Tissue Type: glandular lumen

AGTR1 Antibody Immunohistochemistry validation ( dilution) in glandular lumen (Cat no:25343-1-AP)

{{ptg:RelatedPrimaryAntibodies}}