Anticorps Monoclonal anti-AGTR1

AGTR1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

2C5G11

Avis (1)

N° de cat : 66415-1-Ig

Synonymes

Angiotensin II type-1 receptor, Angiotensin II type 1 receptor, AGTR1B, AGTR1A, AG2S

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Galerie de données de validation

• 

  WB analysis of pig heart using 66415-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66415-1-Ig (AGTR1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 66415-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66415-1-Ig (AGTR1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig skeletal muscle using 66415-1-Ig

  pig skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66415-1-Ig (AGTR1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de porc, tissu cardiaque de rat, tissu de muscle squelettique de porc

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

66415-1-Ig cible AGTR1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AGTR1 Protéine recombinante Ag14461
• Nom complet: angiotensin II receptor, type 1
• Masse moléculaire calculée: 359 aa, 41 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC022447
• Symbole du gène: AGTR1
• Identification du gène (NCBI): 185
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Angiotensin II (Ang II), the main effector molecule of the renin-angiotensin system, exerts its actions mainly via interaction with type-1 angiotensin II receptor (AGTR1, also named as AT1R), thereby contributing to blood pressure regulation. AGTR1 mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. By regulating vascular tone, cardiovascular function, salt and water homeostasis, AGTR1 exerts an indispensable physiological role (PMID: 21600887). AGTR1 has been implicated in diverse aspects of human disease, from the regulation of blood pressure and cardiovascular homeostasis to cancer progression (PMID: 26975580).

Publications

Species	Application	Title
mouse	WB	J Adv Res; Vitamin D/VDR regulates peripheral energy homeostasis via central renin-angiotensin system.; Authors - Han Su
human	WB	Int J Biol Macromol; A novel angiotensin I-converting enzyme inhibitory peptide APPLRP from Grifola frondosa ameliorated the Ang II-induced vascular modeling in zebrafish model by mediating smooth muscle cells; Authors - Tianyuan Song

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anca (Verified Customer) (10-30-2019)

We could detect AT1R from Plazenta, but only by using Chemiluminiscence. Unfortunately, the pozitive control that we used didn't worked.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Plazenta

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