Anticorps Monoclonal anti-AHCY

AHCY Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F9B10

N° de cat : 66019-1-PBS

Synonymes

Adenosylhomocysteinase, AdoHcyase, AHCY, S adenosylhomocysteine hydrolase, SAHH

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Galerie de données de validation

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  WB analysis using 66019-1-Ig (same clone as 66019-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 66019-1-Ig (same clone as 66019-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66019-1-Ig (same clone as 66019-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66019-1-Ig (same clone as 66019-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66019-1-Ig (same clone as 66019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66019-1-Ig (AHCY antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66019-1-Ig (same clone as 66019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66019-1-Ig (AHCY antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66019-1-Ig (same clone as 66019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66019-1-Ig (same clone as 66019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66019-1-Ig (same clone as 66019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66019-1-Ig (same clone as 66019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AHCY antibody (66019-1-Ig, Clone: 1F9B10 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66019-1-PBS cible AHCY dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AHCY Protéine recombinante Ag18036
• Nom complet: S-adenosylhomocysteine hydrolase
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC011606
• Symbole du gène: AHCY
• Identification du gène (NCBI): 191
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).

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