Anticorps Polyclonal de lapin anti-AHR

• Phare
• Validé par KD/KO

AHR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(32)

N° de cat : 28727-1-AP

Synonymes

Class E basic helix-loop-helix protein 76, bHLHe76, Ah receptor

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Galerie de données de validation

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  WB analysis using 28727-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28727-1-AP (AHR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28727-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28727-1-AP (AHR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 28727-1-AP

  WB result of AHR antibody (28727-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AHR transfected HepG2 cells.

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  IP experiment of PC-3 using 28727-1-AP

  IP result of anti-AHR (IP:28727-1-AP, 4ug; Detection:28727-1-AP 1:5000) with PC-3 cells lysate 1480 ug.

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  IP experiment of HepG2 using 28727-1-AP

  IP result of anti-AHR (IP:28727-1-AP, 4ug; Detection:28727-1-AP 1:5000) with HepG2 cells lysate 1360 ug.

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  IHC staining of mouse small intestine using 28727-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28727-1-AP (AHR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 28727-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28727-1-AP (AHR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 28727-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using AHR antibody (28727-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HepG2
• Résultats positifs en IP: cellules PC-3, cellules HepG2
• Résultats positifs en IHC: tissu d'intestin grêle de souris, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 26 publications below
• IHC: See 4 publications below
• IF: See 8 publications below
• IP: See 3 publications below
• ChIP: See 3 publications below

Informations sur le produit

28727-1-AP cible AHR dans les applications de WB, IHC, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AHR Protéine recombinante Ag28935
• Nom complet: aryl hydrocarbon receptor
• Masse moléculaire calculée: 848 aa, 96 kDa
• Poids moléculaire observé: 105-110 kDa
• Numéro d’acquisition GenBank: BC070080
• Symbole du gène: AHR
• Identification du gène (NCBI): 196
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

Publications

Species	Application	Title
mouse	IF	Biomaterials; Biomimetic nanoplatform with microbiome modulation and antioxidant functions ameliorating insulin resistance and pancreatic β-cell dysfunction for T2DM management; Authors - Xiudan Wang
mouse	IP,ChIP	NPJ Regen Med; Aryl hydrocarbon receptor regulates IL-22 receptor expression on thymic epithelial cell and accelerates thymus regeneration; Authors - Jingyi Shen
human	WB,IF,ChIP	Cell Death Discov; IDO1 inhibits ferroptosis by regulating FTO-mediated m6A methylation and SLC7A11 mRNA stability during glioblastoma progression; Authors - Qianting Tian
mouse	WB	J Agric Food Chem; Fructo-oligosaccharides Alleviated Ulcerative Colitis via Gut Microbiota-Dependent Tryptophan Metabolism in Association with Aromatic Hydrocarbon Receptor Activation in Mice; Authors - Chengcheng Yang
human,mouse	WB,ChIP	Biochem Pharmacol; IDO1-mediated AhR activation up-regulates pentose phosphate pathway via NRF2 to inhibit ferroptosis in lung cancer; Authors - Jiani Zhan
human	WB	Eur J Med Res; Potential 'anti-cancer' effects of esketamine on proliferation, apoptosis, migration and invasion in esophageal squamous carcinoma cells; Authors - Chao Li

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