Anticorps Recombinant de lapin anti-AHR

AHR Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

240325G8

N° de cat : 83369-5-PBS

Synonymes

Class E basic helix-loop-helix protein 76, bHLHe76, Ah receptor, 240325G8

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Galerie de données de validation

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  WB analysis using 83369-5-RR (same clone as 83369-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83369-5-RR (AHR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83369-5-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83369-5-RR (same clone as 83369-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83369-5-RR (AHR antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83369-5-PBS in a different storage buffer formulation.

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Informations sur le produit

83369-5-PBS cible AHR dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: AHR Protéine recombinante Ag12193
• Nom complet: aryl hydrocarbon receptor
• Masse moléculaire calculée: 848 aa, 96 kDa
• Poids moléculaire observé: 105-110 kDa
• Numéro d’acquisition GenBank: BC070080
• Symbole du gène: AHR
• Identification du gène (NCBI): 196
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism (PMID:18483242). It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling (PMID:20423157,18540824). It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

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