Anticorps Monoclonal anti-AIDA

• Phare
• Validé par KD/KO

AIDA Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D11A1

N° de cat : 68153-1-PBS

Synonymes

1D11A1, Axin interaction partner and dorsalization antagonist, Axin interactor, dorsalization-associated protein, C1orf80

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Galerie de données de validation

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  WB analysis using 68153-1-Ig (same clone as 68153-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68153-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68153-1-Ig (same clone as 68153-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68153-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 68153-1-Ig (same clone as 68153-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68153-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68153-1-Ig (same clone as 68153-1-PBS)

  WB result of AIDA antibody (68153-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AIDA transfected HeLa cells. This data was developed using the same antibody clone with 68153-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68153-1-Ig (same clone as 68153-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using AIDA antibody (68153-1-Ig, Clone: 1D11A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68153-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68153-1-PBS cible AIDA dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AIDA Protéine recombinante Ag20597
• Nom complet: axin interactor, dorsalization associated
• Masse moléculaire calculée: 306 aa, 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC015535
• Symbole du gène: AIDA
• Identification du gène (NCBI): 64853
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AIDA, also named as C1orf80, acts as a ventralizing factor during embryogenesis. It inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. It's highest expression in the heart and skeletal muscle. AIDA has three isoforms with MW 35 kDa, 16 kDa and 26 kDa.

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