Anticorps Polyclonal de lapin anti-AIF

• Phare
• Validé par KD/KO

AIF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(89)

Avis (2)

N° de cat : 17984-1-AP

Synonymes

AIFM1, PDCD8, EC:1.6.99.-, Apoptosis-inducing factor 1, mitochondrial

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Galerie de données de validation

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  WB analysis of HeLa using 17984-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17984-1-AP (AIF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17984-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17984-1-AP (AIF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17984-1-AP

  WB result of AIF antibody (17984-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AIF transfected HeLa cells.

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  IP experiment of HeLa using 17984-1-AP

  IP result of anti-AIF (IP:17984-1-AP, 3ug; Detection:17984-1-AP 1:2000) with HeLa cells lysate 1320ug.

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  IHC staining of human kidney using 17984-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17984-1-AP (AIF Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 17984-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17984-1-AP (AIF Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse kidney using 17984-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17984-1-AP (AIF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 17984-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17984-1-AP (AIF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 17984-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17984-1-AP (AIF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 17984-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AIF antibody (17984-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu rénal humain, tissu d'estomac de souris, tissu rénal de rat, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 78 publications below
• IHC: See 7 publications below
• IF: See 21 publications below

Informations sur le produit

17984-1-AP cible AIF dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AIF Protéine recombinante Ag12400
• Nom complet: apoptosis-inducing factor, mitochondrion-associated, 1
• Masse moléculaire calculée: 609 aa, 66 kDa
• Poids moléculaire observé: 67 kDa, 57 kDa
• Numéro d’acquisition GenBank: BC111065
• Symbole du gène: AIF
• Identification du gène (NCBI): 9131
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID: 25101006).

Publications

Species	Application	Title
mouse,human	WB	Cell Rep; Newly synthesized AIFM1 determines the hypersensitivity of T lymphocytes to STING activation-induced cell apoptosis; Authors - Wangsheng Ji ; KD Validated
rat	WB	Phytomedicine; LC-MS based metabonomics study on protective mechanism of ESWW in cerebral ischemia via CYTC/Apaf-1/NDRG4 pathway; Authors - Yan Liang
rat	WB	Oxid Med Cell Longev; miRNA-27a Transcription Activated by c-Fos Regulates Myocardial Ischemia-Reperfusion Injury by Targeting ATAD3a.; Authors - Yandong Bao
human	WB	Elife; Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.; Authors - Maxence Le Vasseur
human	WB	PLoS Pathog; GP73 represses host innate immune response to promote virus replication by facilitating MAVS and TRAF6 degradation.; Authors - Xuewu Zhang
human	WB	J Med Chem; Anticancer Effects of Honokiol via Mitochondrial Dysfunction Are Strongly Enhanced by the Mitochondria-Targeting Carrier Berberine.; Authors - Xiaojia Shi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (11-13-2020)

HeLa cells permeablisied (0.1% Triton X-100 in PBS) then blocked (1% BSA in PBS). Cells incubated with primary antibody (1:100) for 1 hour, 3xPBS prior to 1 hour incubation with goat anti-rabbit 488 Alexa Fluor secondary antibody.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa

AIF Antibody Immunofluorescence validation (1:100 dilution) in HeLa (Cat no:17984-1-AP)

FH

Praveen (Verified Customer) (05-17-2019)

Excellent Antibody

• Applications: Western Blot,
• Cell Tissue Type: AC16

AIF Antibody Western Blot, validation ( dilution) in AC16 (Cat no:17984-1-AP)

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