Anticorps Recombinant de lapin anti-AIF

AIF Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250772F8

N° de cat : 86156-3-PBS

Synonymes

AIFM1, Apoptosis-inducing factor 1, mitochondrial, EC:1.6.99.-, PDCD8

---

Galerie de données de validation

• 

  WB analysis using 86156-3-RR (same clone as 86156-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86156-3-RR (AIF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86156-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86156-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86156-3-RR against Human AIF were performed. The affinity constant is 89.1 pM.

---

Informations sur le produit

86156-3-PBS cible AIF dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: AIF Protéine recombinante Ag13268
• Nom complet: apoptosis-inducing factor, mitochondrion-associated, 1
• Masse moléculaire calculée: 609 aa, 66 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC111065
• Symbole du gène: AIF
• Identification du gène (NCBI): 9131
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).

{{ptg:RelatedPrimaryAntibodies}}