Anticorps Polyclonal de lapin anti-AIFM2/ FSP1

• Phare
• Validé par KD/KO

AIFM2/ FSP1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

78

N° de cat : 20886-1-AP

Synonymes

AIFM2, AIFM2/ FSP1, AMID, Apoptosis inducing factor 2, ferroptosis suppressor protein 1, FSP1, p53 responsive gene 3 protein, PRG3, RP11 367H5.2

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Galerie de données de validation

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  WB analysis using 20886-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20886-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20886-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 20886-1-AP

  IP result of anti-AIFM2/ FSP1(IP:20886-1-AP, 4ug; Detection:20886-1-AP 1:3000) with L02 cells lysate 1040 ug.

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  IHC staining of mouse liver using 20886-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 20886-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HeLa, cellules HepG2, tissu hépatique de rat
• Résultats positifs en IP: cellules L02,
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 68 publications below
• IHC: See 12 publications below
• IF: See 11 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

20886-1-AP cible AIFM2/ FSP1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris, duck
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AIFM2/ FSP1 Protéine recombinante Ag13412
• Nom complet: apoptosis-inducing factor, mitochondrion-associated, 2
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC023601
• Symbole du gène: AIFM2
• Identification du gène (NCBI): 84883
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.

Publications

Species	Application	Title
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
human	WB	Nature; The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis.; Authors - Kirill Bersuker
mouse	WB	Nat Commun; Targeting ferroptosis by poly(acrylic) acid coated Mn3O4 nanoparticles alleviates acute liver injury; Authors - Xinyi Shan
mouse	WB	Nat Commun; A targetable LIFR-NF-κB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis.; Authors - Fan Yao
mouse	IHC,IF	Nat Commun; Tartrate-resistant acid phosphatase 5 promotes pulmonary fibrosis by modulating β-catenin signaling.; Authors - Yinan Hu
human	WB	Nat Commun; A targetable CoQ-FSP1 axis drives ferroptosis- and radiation-resistance in KEAP1 inactive lung cancers.; Authors - Pranavi Koppula