Anticorps Polyclonal de lapin anti-AIFM2/ FSP1

• Phare
• Validé par KD/KO

AIFM2/ FSP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20886-1-PBS

Synonymes

AMID, AIFM2, FSP1, EC:1.6.5.-, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death

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Galerie de données de validation

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  WB analysis using 20886-1-AP (same clone as 20886-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

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  WB analysis using 20886-1-AP (same clone as 20886-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

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  WB analysis using 20886-1-AP (same clone as 20886-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

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  IP experiment of L02 using 20886-1-AP (same clone as 20886-1-PBS)

  IP result of anti-AIFM2/ FSP1 (IP:20886-1-AP, 4ug; Detection:20886-1-AP 1:3000) with L02 cells lysate 1040 ug. This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 20886-1-AP (same clone as 20886-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 20886-1-AP (same clone as 20886-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 20886-1-AP (same clone as 20886-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using AIFM2/ FSP1 antibody (20886-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20886-1-PBS cible AIFM2/ FSP1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AIFM2/ FSP1 Protéine recombinante Ag13412
• Nom complet: apoptosis-inducing factor, mitochondrion-associated, 2
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC023601
• Symbole du gène: AIFM2/ FSP1
• Identification du gène (NCBI): 84883
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis-associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis-Inducing Factor), a mitochondrion-associated enzyme that relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.

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