Anticorps Recombinant de lapin anti-AIFM2/ FSP1

AIFM2/ FSP1 Recombinant Antibody for WB, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2J1

N° de cat : 82974-1-PBS

Synonymes

AMID, AIFM2, FSP1, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, Apoptosis inducing factor 2

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Galerie de données de validation

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  WB analysis using 82974-1-RR (same clone as 82974-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82974-1-RR (AIFM2/ FSP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82974-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 82974-1-RR (same clone as 82974-1-PBS)

  IP result of anti-AIFM2/ FSP1 (IP:82974-1-RR, 4ug; Detection:82974-1-RR 1:3000) with HepG2 cells lysate 1085 ug. This data was developed using the same antibody clone with 82974-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 82974-1-RR (same clone as 82974-1-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug AIFM2/ FSP1 Recombinant antibody (82974-1-RR, Clone:2J1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82974-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82974-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82974-1-RR against Human AIFM2/ FSP1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82974-1-PBS cible AIFM2/ FSP1 dans les applications de WB, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: AIFM2/ FSP1 Protéine recombinante Ag13412
• Nom complet: apoptosis-inducing factor, mitochondrion-associated, 2
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC023601
• Symbole du gène: AIFM2/ FSP1
• Identification du gène (NCBI): 84883
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.

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