Anticorps Polyclonal de lapin anti-AIG1

AIG1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 14468-1-AP

Synonymes

AIG 1, AIG1, androgen induced 1

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Galerie de données de validation

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  WB analysis using 14468-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 14468-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of mouse heart using 14468-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of mouse testis using 14468-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14468-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of rat ovary using 14468-1-AP

  rat ovary tissue were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 14468-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 14468-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14468-1-AP (AIG1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary tumor using 14468-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14468-1-AP (AIG1 antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules PC-3, tissu cardiaque de rat, tissu cardiaque de souris, tissu ovarien de rat, tissu ovarien de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

14468-1-AP cible AIG1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AIG1 Protéine recombinante Ag5836
• Nom complet: androgen-induced 1
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28-32 kDa
• Numéro d’acquisition GenBank: BC025278
• Symbole du gène: AIG1
• Identification du gène (NCBI): 51390
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
	WB	Nat Commun; Inflammation causes insulin resistance in mice via interferon regulatory factor 3 (IRF3)-mediated reduction in FAHFA levels; Authors - Shuai Yan

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