- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-AIM2
AIM2 Polyclonal Antibody for WB, IHC, IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain et plus (3)
Applications
WB, IHC, IP, ELISA
Conjugaison
Non conjugué
N° de cat : 20590-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules A431, cellules A549, cellules HeLa, cellules HepG2, cellules MCF-7, cellules Raji |
| Résultats positifs en IP | cellules Raji, |
| Résultats positifs en IHC | tissu testiculaire humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF/ICC | cellules MCF-7, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochimie (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 5 publications below |
| WB | See 29 publications below |
| IHC | See 11 publications below |
Informations sur le produit
20590-1-AP cible AIM2 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Réactivité citée | rat, Humain, poisson-zèbre, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | AIM2 Protéine recombinante Ag14520 |
| Nom complet | absent in melanoma 2 |
| Masse moléculaire calculée | 343 aa, 39 kDa |
| Poids moléculaire observé | 39-42 kDa |
| Numéro d’acquisition GenBank | BC010940 |
| Symbole du gène | AIM2 |
| Identification du gène (NCBI) | 9447 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria (PMID:22695634). Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity (PMID:16432157,20566831). It has been reported that AIM2 protein levels are significantly elevated in alveolar macrophages of patients with IPF and bleomycin-treated mice (PMID: 38078661). AIM2 inflammasome was also significantly activated, which was also confirmed in Aβ-treated microglial cells (PMID: 34861878)
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for AIM2 antibody 20590-1-AP | Download protocol |
| IHC protocol for AIM2 antibody 20590-1-AP | Download protocol |
| IF protocol for AIM2 antibody 20590-1-AP | Download protocol |
| IP protocol for AIM2 antibody 20590-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Mol Immunol NLRP3-dependent pyroptosis is required for HIV-1 gp120-induced neuropathology.
| ||
Mucosal Immunol AIM2 contributes to the maintenance of intestinal integrity via Akt and protects against Salmonella mucosal infection. | ||
J Agric Food Chem Integrative Gut Microbiota and Metabolomic Analyses Reveal the PANoptosis- and Ferroptosis-Related Mechanisms of Chrysoeriol in Inhibiting Melanoma | ||
Front Immunol Cytokine Secretion and Pyroptosis of Thyroid Follicular Cells Mediated by Enhanced NLRP3, NLRP1, NLRC4, and AIM2 Inflammasomes Are Associated With Autoimmune Thyroiditis. | ||
Front Cell Dev Biol Autophagy-Based Unconventional Secretory for AIM2 Inflammasome Drives DNA Damage Resistance During Intervertebral Disc Degeneration.
| ||
Front Immunol Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma |





