Anticorps Monoclonal anti-AIM2

• Phare
• Validé par KD/KO

AIM2 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(19)

CloneNo.

3E12A8

N° de cat : 66902-1-Ig

Synonymes

PYHIN4, Interferon-inducible protein AIM2, 3E12A8

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Galerie de données de validation

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  WB analysis using 66902-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit spleen using 66902-1-Ig

  rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66902-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 66902-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human urothelial carcinoma using 66902-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 66902-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat small intestine using 66902-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, cellules Sp2/0, tissu cardiaque humain, tissu splénique de lapin
• Résultats positifs en IHC: tissu de carcinome urothélial humain, tissu d'intestin grêle de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

66902-1-Ig cible AIM2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AIM2 Protéine recombinante Ag14607
• Nom complet: absent in melanoma 2
• Masse moléculaire calculée: 343 aa, 39 kDa
• Poids moléculaire observé: 37-39 and 53 kDa
• Numéro d’acquisition GenBank: BC010940
• Symbole du gène: AIM2
• Identification du gène (NCBI): 9447
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID:22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]

Publications

Species	Application	Title
human	CoIP	Gut; STAT3-mediated upregulation of the AIM2 DNA sensor links innate immunity with cell migration to promote epithelial tumourigenesis.; Authors - Ruby E Dawson
human	WB	Theranostics; Establishment of tumor inflammasome clusters with distinct immunogenomic landscape aids immunotherapy; Authors - Qingyu Liang
mouse	WB	Inflamm Res; Transcriptomic analysis reveals molecular characterization and immune landscape of PANoptosis-related genes in atherosclerosis; Authors - Zhipeng Zheng
human	WB	Front Oncol; PKIB involved in the metastasis and survival of osteosarcoma; Authors - Rongxue Wan
mouse	WB	Cell Commun Signal; Increased susceptibility of irradiated mice to Aspergillus fumigatus infection via NLRP3/GSDMD pathway in pulmonary bronchial epithelia.; Authors - Dong-Ming Wu
human	WB	Cell Death Discov; Tumors cells with mismatch repair deficiency induce hyperactivation of pyroptosis resistant to cell membrane damage but are more sensitive to co-treatment of IFN-γ and TNF-α to PANoptosis; Authors - Huiyan Li

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