Anticorps Monoclonal anti-AIM2

• Phare
• Validé par KD/KO

AIM2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3E12A8

N° de cat : 66902-1-PBS

Synonymes

PYHIN4, Interferon-inducible protein AIM2, 3E12A8

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Galerie de données de validation

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  WB analysis using 66902-1-Ig (same clone as 66902-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit spleen using 66902-1-Ig (same clone as 66902-1-PBS)

  rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66902-1-Ig (same clone as 66902-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66902-1-Ig (same clone as 66902-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 66902-1-Ig (same clone as 66902-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 66902-1-Ig (same clone as 66902-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

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  IHC staining of rat small intestine using 66902-1-Ig (same clone as 66902-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66902-1-PBS cible AIM2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AIM2 Protéine recombinante Ag14607
• Nom complet: absent in melanoma 2
• Masse moléculaire calculée: 343 aa, 39 kDa
• Poids moléculaire observé: 37-39 and 53 kDa
• Numéro d’acquisition GenBank: BC010940
• Symbole du gène: AIM2
• Identification du gène (NCBI): 9447
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID:22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]

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