Anticorps Monoclonal anti-AIRE

AIRE Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A3C3

N° de cat : 66262-1-PBS

Synonymes

PGA1, APSI, APS1, APECED, AIRE1

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Galerie de données de validation

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  WB analysis of human spleen using 66262-1-Ig (same clone as 66262-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66262-1-Ig (AIRE Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66262-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig spleen using 66262-1-Ig (same clone as 66262-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66262-1-Ig (AIRE Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66262-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 66262-1-Ig (same clone as 66262-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66262-1-Ig (AIRE Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66262-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66262-1-Ig (same clone as 66262-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AIRE antibody (66262-1-Ig, Clone: 1A3C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66262-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66262-1-PBS cible AIRE dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AIRE Protéine recombinante Ag19103
• Nom complet: autoimmune regulator
• Masse moléculaire calculée: 545 aa, 58 kDa
• Poids moléculaire observé: 58-69 kDa
• Numéro d’acquisition GenBank: BC137270
• Symbole du gène: AIRE
• Identification du gène (NCBI): 326
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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