Anticorps Polyclonal de lapin anti-AK1

AK1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14978-1-PBS

Synonymes

adenylate kinase 1, Adenylate kinase isoenzyme 1, Adenylate monophosphate kinase, AK 1, ATP:AMP phosphotransferase

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Galerie de données de validation

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  WB analysis using 14978-1-AP (same clone as 14978-1-PBS)

  mouse heart lysates were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14978-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14978-1-AP (same clone as 14978-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14978-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 14978-1-AP (same clone as 14978-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14978-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 14978-1-AP (same clone as 14978-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14978-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 14978-1-AP (same clone as 14978-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14978-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 14978-1-AP (same clone as 14978-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14978-1-AP (AK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14978-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 14978-1-AP (same clone as 14978-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14978-1-AP (AK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14978-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14978-1-PBS cible AK1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AK1 Protéine recombinante Ag6890
• Nom complet: adenylate kinase 1
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC001116
• Symbole du gène: AK1
• Identification du gène (NCBI): 203
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AK1(Adenylate kinase isoenzyme 1), also known as ATP-AMP transphosphorylase 1. It is located in cytoplasm. This protein is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP 1. Exhibits nucleoside diphosphate kinase activity, catalyzing the production of ATP, CTP, GTP, UTP, dATP, dCTP, dGTP and dTTP from the corresponding diphosphate substrates with either ATP or GTP as phosphate donor 2. Also catalyzes at a very low rate the synthesis of thiamine triphosphate (ThTP) from thiamine diphosphate (ThDP) and ADP. The molecular weight of AK1 is 22 kDa.

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