Anticorps Recombinant de lapin anti-AK1

AK1 Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

250557D10

N° de cat : 85979-3-PBS

Synonymes

adenylate kinase 1, Adenylate kinase isoenzyme 1, Adenylate monophosphate kinase, AK 1, ATP:AMP phosphotransferase

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Galerie de données de validation

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  WB analysis using 85979-3-RR (same clone as 85979-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85979-3-RR (AK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85979-3-PBS in a different storage buffer formulation.

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  WB analysis using 85979-3-RR (same clone as 85979-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85979-3-RR (AK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85979-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02232-2

  Sandwich ELISA standard curve of MP02232-2, Human AK1 Recombinant Matched Antibody Pair - PBS only. 85979-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6890. 85979-3-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP02232-2

  The mean AK1 concentration was determined to be 35.20 ng/mL in HeLa cell extract based on a 0.9 mg/mL extract load.

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  Sample test of MP02232-2

  Human milk of six individual healthy human donors was measured. The human AK1 concentration of detected samples was determined to be 2.83 ng/mL with a range of 0.76 - 8.63 ng/mL.

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  Affinity and Kinetic Characterization of 85979-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85979-3-RR against Human AK1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

85979-3-PBS cible AK1 dans les applications de WB, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: AK1 Protéine recombinante Ag6890
• Nom complet: adenylate kinase 1
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC001116
• Symbole du gène: AK1
• Identification du gène (NCBI): 203
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AK1(Adenylate kinase isoenzyme 1), also known as ATP-AMP transphosphorylase 1. It is located in cytoplasm. This protein is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP 1. Exhibits nucleoside diphosphate kinase activity, catalyzing the production of ATP, CTP, GTP, UTP, dATP, dCTP, dGTP and dTTP from the corresponding diphosphate substrates with either ATP or GTP as phosphate donor 2. Also catalyzes at a very low rate the synthesis of thiamine triphosphate (ThTP) from thiamine diphosphate (ThDP) and ADP. The molecular weight of AK1 is 22 kDa.

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