Anticorps Recombinant de lapin anti-AK1

AK1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250557D10

N° de cat : 85979-3-RR

Synonymes

adenylate kinase 1, Adenylate kinase isoenzyme 1, Adenylate monophosphate kinase, AK 1, ATP:AMP phosphotransferase

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Galerie de données de validation

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  WB analysis using 85979-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85979-3-RR (AK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85979-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85979-3-RR (AK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85979-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85979-3-RR against Human AK1 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HeLa, cellules K-562, tissu cardiaque de rat, tissu cardiaque humain

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85979-3-RR cible AK1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: AK1 Protéine recombinante Ag6890
• Nom complet: adenylate kinase 1
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC001116
• Symbole du gène: AK1
• Identification du gène (NCBI): 203
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AK1(Adenylate kinase isoenzyme 1), also known as ATP-AMP transphosphorylase 1. It is located in cytoplasm. This protein is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP 1. Exhibits nucleoside diphosphate kinase activity, catalyzing the production of ATP, CTP, GTP, UTP, dATP, dCTP, dGTP and dTTP from the corresponding diphosphate substrates with either ATP or GTP as phosphate donor 2. Also catalyzes at a very low rate the synthesis of thiamine triphosphate (ThTP) from thiamine diphosphate (ThDP) and ADP. The molecular weight of AK1 is 22 kDa.

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