Anticorps Polyclonal de lapin anti-AK2

AK2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 11014-1-AP

Synonymes

ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferase, ADK2, Adenylate monophosphate kinase, Adenylate kinase 2, mitochondrial, N-terminally processed

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Galerie de données de validation

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  WB analysis using 11014-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11014-1-AP (AK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 11014-1-AP

  IP result of anti-AK2 (IP:11014-1-AP, 3ug; Detection:11014-1-AP 1:800) with mouse liver tissue lysate 12500ug.

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  IHC staining of human pancreas cancer using 11014-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11014-1-AP (AK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11014-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11014-1-AP (AK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11014-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AK2 antibody (11014-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HeLa, cellules HepG2, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

11014-1-AP cible AK2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AK2 Protéine recombinante Ag1464
• Nom complet: adenylate kinase 2
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC009405
• Symbole du gène: AK2
• Identification du gène (NCBI): 204
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.

Publications

Species	Application	Title
zebrafish	IHC	Nat Genet; Reticular dysgenesis (aleukocytosis) is caused by mutations in the gene encoding mitochondrial adenylate kinase 2.; Authors - Pannicke Ulrich U
human	WB,IF	Blood; The palmitoyltransferase ZDHHC21 regulates oxidative phosphorylation to induce differentiation block and stemness in AML; Authors - Xuejing Shao
human	WB	Neuron; Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.; Authors - Nathalie Escande-Beillard
human	WB	J Exp Med; A loss-of-function NUAK2 mutation in humans causes anencephaly due to impaired Hippo-YAP signaling.; Authors - Carine Bonnard
human	WB	EMBO Mol Med; A progeroid syndrome caused by a deep intronic variant in TAPT1 is revealed by RNA/SI-NET sequencing; Authors - Nasrinsadat Nabavizadeh
human	WB,IF	Fluids Barriers CNS; Cocaine regulates antiretroviral therapy CNS access through pregnane-x receptor-mediated drug transporter and metabolizing enzyme modulation at the blood brain barrier; Authors - Rodnie Colón Ortiz

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