Anticorps Polyclonal de lapin anti-AK3

AK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12562-1-PBS

Synonymes

Adenylate kinase 3 alpha-like 1, AK 3, AK3L1, AK6, AKL3L

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Galerie de données de validation

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  WB analysis using 12562-1-AP (same clone as 12562-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 12562-1-AP (same clone as 12562-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 12562-1-AP (same clone as 12562-1-PBS)

  IP result of anti-AK3 (IP:12562-1-AP, 3ug; Detection:12562-1-AP 1:500) with mouse kidney tissue lysate 4400ug. This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 12562-1-AP (same clone as 12562-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12562-1-AP (AK3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 12562-1-AP (same clone as 12562-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12562-1-AP (AK3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 12562-1-AP (same clone as 12562-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12562-1-AP (AK3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12562-1-AP (same clone as 12562-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AK3 antibody (12562-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12562-1-PBS cible AK3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AK3 Protéine recombinante Ag3146
• Nom complet: adenylate kinase 3
• Masse moléculaire calculée: 227 aa, 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC013771
• Symbole du gène: AK3
• Identification du gène (NCBI): 50808
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AK3(Adenylate kinase 3) is also named as AK3L1, AK6, AKL3L and belongs to the adenylate kinase family. It is located in the mitochondrial matrix and is a GTP:AMP phosphotransferase(PMID:2546792). AK3 can be detected AK3 at an apparent molecular mass of 28 kD by western blot analysis and subcellular fractionation of mouse liver and kidney detected Ak3 in the mitochondrial fraction(PMID:11485571). This antibody may also recognize AK4 due to the high homology.

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