Anticorps Polyclonal de lapin anti-AKAP1

AKAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15618-1-PBS

Synonymes

D-AKAP-1, DAKAP1, A-kinase anchor protein 149 kDa, A-kinase anchor protein 1, mitochondrial, AKAP 1

---

Galerie de données de validation

• 

  WB analysis using 15618-1-AP (same clone as 15618-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15618-1-AP (AKAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 15618-1-AP (same clone as 15618-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 15618-1-AP (AKAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

• 

  IP experiment of PC-3 using 15618-1-AP (same clone as 15618-1-PBS)

  IP result of anti-AKAP1 (IP:15618-1-AP, 4ug; Detection:15618-1-AP 1:2000) with PC-3 cells lysate 1800ug. This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 15618-1-AP (same clone as 15618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15618-1-AP (AKAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 15618-1-AP (same clone as 15618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15618-1-AP (AKAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 15618-1-AP (same clone as 15618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15618-1-AP (AKAP1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 15618-1-AP (same clone as 15618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15618-1-AP (AKAP1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 15618-1-AP (same clone as 15618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15618-1-AP (AKAP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 15618-1-AP (same clone as 15618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15618-1-AP (AKAP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 15618-1-AP (same clone as 15618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15618-1-AP (AKAP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 15618-1-AP (same clone as 15618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15618-1-AP (AKAP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 15618-1-AP (same clone as 15618-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using AKAP1 antibody 15618-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

15618-1-PBS cible AKAP1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AKAP1 Protéine recombinante Ag8037
• Nom complet: A kinase (PRKA) anchor protein 1
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 149 kDa
• Numéro d’acquisition GenBank: BC000729
• Symbole du gène: AKAP1
• Identification du gène (NCBI): 8165
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AKAP1 (also termed AKAP149) is a human 149 kDa anchoring protein localized in mitochondria and the endoplasmic reticulum/nuclear envelope (ER-NE) network. It Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment.

{{ptg:RelatedPrimaryAntibodies}}