Anticorps Recombinant de lapin anti-AKAP1

AKAP1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242763A5

N° de cat : 85119-2-PBS

Synonymes

242763A5, AKAP, AKAP 1, A-kinase anchor protein 1, mitochondrial, A-kinase anchor protein 149 kDa

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Galerie de données de validation

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  WB analysis using 85119-2-RR (same clone as 85119-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85119-2-RR (AKAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85119-2-RR (same clone as 85119-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85119-2-RR (AKAP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85119-2-RR (same clone as 85119-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85119-2-RR (AKAP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85119-2-RR (same clone as 85119-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AKAP1 antibody (85119-2-RR, Clone: 242763A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 85119-2-RR (same clone as 85119-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using AKAP1 antibody (85119-2-RR, Clone: 242763A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293T using 85119-2-RR (same clone as 85119-2-PBS)

  1x10^6 HEK-293T cells were intracellularly stained with 0.13 ug AKAP1 Recombinant antibody (85119-2-RR, Clone:242763A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85119-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85119-2-RR against Human AKAP1 were performed. The affinity constant is 3.62 nM.

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Informations sur le produit

85119-2-PBS cible AKAP1 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: AKAP1 Protéine recombinante Ag8037
• Nom complet: A kinase (PRKA) anchor protein 1
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 149 kDa
• Numéro d’acquisition GenBank: BC000729
• Symbole du gène: AKAP1
• Identification du gène (NCBI): 8165
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AKAP1 (also termed AKAP149) is a human 149 kDa anchoring protein localized in mitochondria and the endoplasmic reticulum/nuclear envelope (ER-NE) network. It Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment.

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