Anticorps Polyclonal de lapin anti-AKAP12

AKAP12 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (2)

N° de cat : 25199-1-AP

Synonymes

A kinase anchor protein 12, AKAP 12, AKAP 250, AKAP12, AKAP250, Gravin, Myasthenia gravis autoantigen

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Galerie de données de validation

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  WB analysis using 25199-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25199-1-AP (AKAP12 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 25199-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25199-1-AP (AKAP12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 25199-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25199-1-AP (AKAP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25199-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25199-1-AP (AKAP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 25199-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25199-1-AP (AKAP12 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 25199-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25199-1-AP (AKAP12 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat,
• Résultats positifs en IHC: tissu cérébral de souris, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

25199-1-AP cible AKAP12 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AKAP12 Protéine recombinante Ag17769
• Nom complet: A kinase (PRKA) anchor protein 12
• Masse moléculaire calculée: 1782 aa, 191 kDa
• Poids moléculaire observé: 200-300 kDa
• Numéro d’acquisition GenBank: BC157067
• Symbole du gène: AKAP12
• Identification du gène (NCBI): 9590
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AKAP12, also named as AKAP250, is a 1782 amino acid protein, which contains three AKAP domains. AKAP12 localizes in the cytoplasm and is expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells. AKAP12 as an anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC). AKAP12 expression is known to be suppressed in several human malignancies including breast, prostate, gastric and colon cancers. AKAP12 has three isoforms, namely AKAP12A, AKAP12B and AKAP12C, with molecular weights of 305 kDa, 287 kDa and 250 kDa respectively. (PMID: 30385176, PMID: 15258566)

Publications

Species	Application	Title
human	IHC	J Transl Med; Prognostic risk factors of serous ovarian carcinoma based on mesenchymal stem cell phenotype and guidance for therapeutic efficacy; Authors - Xiaohui Yang
human	WB	Mol Ther Nucleic Acids; The Human Novel Gene LNC-HC Inhibits Hepatocellular Carcinoma Cell Proliferation by Sequestering hsa-miR-183-5p.; Authors - Xi Lan
human	WB,IHC	Front Endocrinol (Lausanne); Construction of novel hypoxia-related gene model for prognosis and tumor microenvironment in endometrial carcinoma; Authors - Junfeng Chen
mouse	IF	Sci Rep; β-catenin regulates the formation of multiple nephron segments in the mouse kidney.; Authors - Patrick Deacon
mouse	IF	Surgery; Heat shock protein A12B gene therapy improves perfusion, promotes neovascularization, and decreases fibrosis in a murine model of hind limb ischemia.; Authors - Mahesh Thirunavukkarasu
human	WB	Am J Transl Res; Comprehensive analysis of differentially expressed non-coding RNAs and mRNAs in gastric cancer cells under hypoxic conditions.; Authors - Jia Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Pradeep (Verified Customer) (07-20-2020)

I used for immunofluorescence in mouse tissue sample. it is working great.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse HLI

FH

Rushita (Verified Customer) (11-26-2019)

The Ab works very well for detection of endogenous AKAP12 for western blotting (1:1000) in HEK 293 and COS7 cell lines.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293, COS7