Anticorps Polyclonal de lapin anti-AKAP12

• Phare
• Validé par KD/KO

AKAP12 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25199-1-PBS

Synonymes

A kinase anchor protein 12, AKAP 12, AKAP 250, AKAP-12, AKAP250

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Galerie de données de validation

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  WB analysis using 25199-1-AP (same clone as 25199-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25199-1-AP (AKAP12 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 25199-1-AP (same clone as 25199-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25199-1-AP (AKAP12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 25199-1-AP (same clone as 25199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25199-1-AP (AKAP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 25199-1-AP (same clone as 25199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25199-1-AP (AKAP12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 25199-1-AP (same clone as 25199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25199-1-AP (AKAP12 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 25199-1-AP (same clone as 25199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25199-1-AP (AKAP12 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25199-1-PBS cible AKAP12 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AKAP12 Protéine recombinante Ag17769
• Nom complet: A kinase (PRKA) anchor protein 12
• Masse moléculaire calculée: 1782 aa, 191 kDa
• Poids moléculaire observé: 200-300 kDa
• Numéro d’acquisition GenBank: BC157067
• Symbole du gène: AKAP12
• Identification du gène (NCBI): 9590
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AKAP12, also named as AKAP250, is a 1782 amino acid protein, which contains three AKAP domains. AKAP12 localizes in the cytoplasm and is expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells. AKAP12 as an anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC). AKAP12 expression is known to be suppressed in several human malignancies including breast, prostate, gastric and colon cancers. AKAP12 has three isoforms, namely AKAP12A, AKAP12B and AKAP12C, with molecular weights of 305 kDa, 287 kDa and 250 kDa respectively. (PMID: 30385176, PMID: 15258566)

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