Anticorps Polyclonal de lapin anti-AKAP13

AKAP13 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27947-1-PBS

Synonymes

A kinase anchor protein 13, AKAP 13, A-kinase anchor protein 13, ARHGEF13, BRX

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Galerie de données de validation

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  WB analysis using 27947-1-AP (same clone as 27947-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 27947-1-AP (AKAP13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 27947-1-AP (same clone as 27947-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 27947-1-AP (same clone as 27947-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 27947-1-AP (same clone as 27947-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 27947-1-AP (same clone as 27947-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 27947-1-AP (same clone as 27947-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 27947-1-AP (same clone as 27947-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27947-1-PBS cible AKAP13 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AKAP13 Protéine recombinante Ag27486
• Nom complet: A kinase (PRKA) anchor protein 13
• Masse moléculaire calculée: 308 kDa
• Poids moléculaire observé: 308 kDa
• Numéro d’acquisition GenBank: NM_001270546
• Symbole du gène: AKAP13
• Identification du gène (NCBI): 11214
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms containing c-terminal dbl oncogene homology (DH) and pleckstrin homology (PH) domains. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins, resulting in the conversion of the inactive GTPase to the active form capable of transducing signals. The PH domain has multiple functions

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