Anticorps Monoclonal anti-AKAP3

AKAP3 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3C11H9

N° de cat : 66255-1-PBS

Synonymes

A kinase anchor protein 3, AKAP 110, AKAP 3, AKAP110, AKAP3, Cancer/testis antigen 82, CT82, Fibrousheathin 1, Fibrousheathin I, FSP95, PRKA3, SOB1, Sperm oocyte binding protein

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Galerie de données de validation

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  WB analysis of human testis using 66255-1-Ig (same clone as 66255-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66255-1-Ig (AKAP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66255-1-Ig (AKAP3 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66255-1-Ig (AKAP3 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AKAP3 antibody (66255-1-Ig, Clone: 3C11H9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AKAP3 antibody (66255-1-Ig, Clone: 3C11H9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66255-1-PBS cible AKAP3 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AKAP3 Protéine recombinante Ag4885
• Nom complet: A kinase (PRKA) anchor protein 3
• Masse moléculaire calculée: 95 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC047535
• Symbole du gène: AKAP3
• Identification du gène (NCBI): 10566
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.

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